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t47d kbluc cell line  (ATCC)


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    ATCC t47d kbluc cell line
    T47d Kbluc Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t47d+kbluc+cell+line/T47D-KBluc/pmc12722357-72-2-6
    Average 95 stars, based on 183 article reviews
    t47d kbluc cell line - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection ( ATCC ); CRL-2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) ( ) or human GR (Ad/GR4) ( ) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) ( ) into CV-1 cells ( ATCC CCL-70) to assess GR and AR activity, respectively.

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection (ATCC); CRL2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) (Hartig et al. 2007) or human GR (Ad/ GR4) (Shih et al. 1991) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) (Shih et al. 1991) into CV-1 cells (ATCC CCL-70) to assess GR and AR activity, respectively.

    Stable Transfection:

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection ( ATCC ); CRL-2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) ( ) or human GR (Ad/GR4) ( ) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) ( ) into CV-1 cells ( ATCC CCL-70) to assess GR and AR activity, respectively.

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection (ATCC); CRL2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) (Hartig et al. 2007) or human GR (Ad/ GR4) (Shih et al. 1991) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) (Shih et al. 1991) into CV-1 cells (ATCC CCL-70) to assess GR and AR activity, respectively.

    Transfection:

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection ( ATCC ); CRL-2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) ( ) or human GR (Ad/GR4) ( ) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) ( ) into CV-1 cells ( ATCC CCL-70) to assess GR and AR activity, respectively.

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection (ATCC); CRL2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) (Hartig et al. 2007) or human GR (Ad/ GR4) (Shih et al. 1991) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) (Shih et al. 1991) into CV-1 cells (ATCC CCL-70) to assess GR and AR activity, respectively.

    Modification:

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection ( ATCC ); CRL-2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) ( ) or human GR (Ad/GR4) ( ) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) ( ) into CV-1 cells ( ATCC CCL-70) to assess GR and AR activity, respectively.

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection (ATCC); CRL2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) (Hartig et al. 2007) or human GR (Ad/ GR4) (Shih et al. 1991) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) (Shih et al. 1991) into CV-1 cells (ATCC CCL-70) to assess GR and AR activity, respectively.

    Cell Culture:

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection ( ATCC ); CRL-2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) ( ) or human GR (Ad/GR4) ( ) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) ( ) into CV-1 cells ( ATCC CCL-70) to assess GR and AR activity, respectively.

    Article Title: Adverse Maternal, Fetal, and Postnatal Effects of Hexafluoropropylene Oxide Dimer Acid (GenX) from Oral Gestational Exposure in Sprague-Dawley Rats
    Article Snippet: .. Briefly, for ER activity we utilized the stably transfected T47D-KBluc cell line [publicly available via American Type Culture Collection (ATCC); CRL2865] according to protocols provided by ATCC with the modification of Dulbecco’s Modified Eagle Media (DMEM) as the cell culture media instead of Roswell Park Memorial Institute (RPMI) media. .. We utilized adenoviral transduction to introduce chimp AR (Ad5chAR-g) (Hartig et al. 2007) or human GR (Ad/ GR4) (Shih et al. 1991) and a luciferase-based promoter-reporter construct (MMTV-Luc; Ad/mLuc7) (Shih et al. 1991) into CV-1 cells (ATCC CCL-70) to assess GR and AR activity, respectively.

    Article Title: Exposures and potential health implications of contaminant mixtures in linked source water, finished drinking water, and tapwater from public-supply drinking water systems in Minneapolis/St. Paul area, USA
    Article Snippet: .. Tapwater samples were also analyzed for in vitro estrogen (ER), androgen (AR), and glucocorticoid (GR) bioactivities by EPA using the T47D-kBluc cell line (American Type Cell Culture, Manassas, Virginia; ATCC CRL-2865; human estrogen receptor α/β) and the CV1 cell line (ATCC CCL-70) transduced (adenovirus) with the chimpanzee androgen receptor or the human glucocorticoid receptor as described in previously published methods. ..

    Derivative Assay:

    Article Title: Soft plastic fishing lures as a potential source of chemical pollution - Chemical analyses, toxicological relevance, and anglers' perspectives.
    Article Snippet: .. The T47D-KBluc cell line (ATCC #CRL-2865) was derived from T47D human breast carcinoma cells and expresses endogenous α and β estrogen receptors (Wilson et al., 2004). ..

    In Vitro:

    Article Title: Exposures and potential health implications of contaminant mixtures in linked source water, finished drinking water, and tapwater from public-supply drinking water systems in Minneapolis/St. Paul area, USA
    Article Snippet: .. Tapwater samples were also analyzed for in vitro estrogen (ER), androgen (AR), and glucocorticoid (GR) bioactivities by EPA using the T47D-kBluc cell line (American Type Cell Culture, Manassas, Virginia; ATCC CRL-2865; human estrogen receptor α/β) and the CV1 cell line (ATCC CCL-70) transduced (adenovirus) with the chimpanzee androgen receptor or the human glucocorticoid receptor as described in previously published methods. ..



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